Mapping of ben genes of Pseudomonas aeruginosa.
نویسندگان
چکیده
Four ben genes responsible for the conversion of benzoate to catechol in Pseudomonas aeruginosa PAO have been mapped to a 4.6 kb KpnI fragment, ben-1 and ben-4 were known to be separate genes but now ben-1508 has been found to be different from ben-2. The two genes were distinguished by Tn5 mutagenesis of a cosmid clone and deletion mapping. It is likely that the four genes mapped (ben-4, ben-2, ben-1508 and ben-1) correspond to the previously characterized benR (regulatory gene) and benABC (benzoate dioxygenase) respectively.
منابع مشابه
Mapping of the ben, ant and cat genes of Pseudomonas aeruginosa and evolutionary relationship of the ben region of P. aeruginosa and P. putida.
Genes responsible for the utilization of benzoate, anthranilate or catechol (ben, ant, cat) of Pseudomonas aeruginosa PAO were mapped precisely using a cosmid clone carrying all these genes. Genes were localized either by subcloning and complementation or by Tn5 mutagenesis and mapping of the Tn5 insertions. To achieve this, a novel Tn5 mutagenesis procedure was developed by constructing a Tn5 ...
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ورودعنوان ژورنال:
- FEMS microbiology letters
دوره 112 3 شماره
صفحات -
تاریخ انتشار 1993